Founded in 1968, this museum in Zyndranowa is an open-air style exposition showcasing Lemko-style homesteads that date to the late 1800s. Here you can view traditional clothes and everyday objects, agricultural tools and old furtniture related to Lemko heritage and culture. One room has a display of religious artefacts including parts of cast iron crucifixes, liturgical garments and replicas of icons. There are also exhibits connected with warfare operations at the Dukla Pass: military uniforms, helmets, weapons and shrapnel shells.

The original founder of this museum was Teodor (Fedor) Gocz, who in the 1950s with his great-grandfather Teodor Kukieła undertook efforts to protect the heritage of the Lemkos who were expelled from Zyndranowa after the war. Although he avoided deportation as part of Operation Vistula, he was repressed for his noble activities. The museum is the result of his efforts to salvage fragments of Lemko culture from these brutal times. Currently, the museum is run by the Society for the Development of Lemko Culture Museum in Zyndranowa, and its activities, after Teodor Gocz's death in 2018, are managed by his son, Bogdan.

For the past 30 years the museum has hosted the Festival of Lemko Traditions at the Borderland of Cultures called “Od Rusal do Jana”.

The museum can be reached along the green trail leading from Barwinek towards Stasien and the Hermitage of Jan of Dukla.